2014 – Electrace: 

Best Microfluidics

Electrace: Project Electrace consisted of three parallel Modules, termed Electron Transport, Conductive Curli and Landmine Detection. Of fundamental interest was the Module Electron Transport. Team iGEM 2014 TU Delft  integrated the Mtr system of metal-reducing bacterium Shewanella oneidensis in E. coli. Upon induction, the transmembrane MtrCAB protein complex formed, which enabled the bacterium to transport electrons to the extracellular environment, generating a measurable current output. To optimize this system, the intricate protein complex formation process was modeled in Deterministic Model of EET Complex Assembly . Also, Flux Balance Analysis was employed to increase generation of electrons and thus transport via E. coli carbon metabolism.