Although a lot of experiments are being done in the lab. There is still a lot of science we can do or need to do outside of the lab. This year the iGEM TU Delft dry lab team will use computational and mathematical methods to simulate and understand natural phenomena studied in the wet lab. Furthermore we will also be working on software to analyse results in the wet lab. The dry lab team will be working hard on four different modules.
For the first module we will be working on a sequence analysis pipeline. The sequence analysis pipeline will be used to determine whether there are possible biosensor sequences in the libraries we have been enriching in the wet lab. The possible biosensor sequences we will find after running the raw data through the pipeline will then be tested out in the wet lab, to see whether they are truly biosensors.
For the second module we will be creating mathematical models of the cell free systems used in the lab. These models are mathematical representations of real world systems and use parameters that are either experimentally determined in our own lab or from literature. These models help us understand how our systems work and we will use them to check whether our system with the aptamers works with aptamers that have a natural binding affinity.
The cell free systems with the aptamers need to have a support for them to work and to be used in the detection kit. This year we aim to use paper as a support. For the third module we will be working on this paper fluidics. We will be trying out different designs for our paper fluidic chips and gathering data to refine and improve our design.
Once we have the paper fluidic chip we are not yet finished. We need to have a device that can actually read out the product concentration. To do this we will build a device that we can use for the readout of our paper fluidic chips. We will work in close collaboration with the human practices team to determine the design requirements for this device.